Vaʻaiga lautele Lactoperoxidase

Lactoperoxidase (LPO), o loʻo maua i totoga faʻalu ma susu susu, o se mea taua tele i le tali atu e puipuia ai le taua i le tausia lelei o le soifua maloloina lelei. Ole vaega taua o le lactoperoxidase o le faʻaʻofiofia o ion thiocyanate (SCN−) o loʻo maua i totonu o fuluʻi i luma o le hydrogen peroxide mafuaʻaga o oloa e faʻaalia ai gaioiga antimicrobial. LPO o loʻo maua i le susu povi ua faʻaaogaina i le fomai, meaʻai ma mea teuteu teuteu ona o lona aoga ma fausaga tutusa ma le enzyme o le tagata.

O loʻo faʻamaninoina i le lactoperoxidase faiga o loʻo faʻaogaina nei mea e faʻaaoga ai se vailaʻau mo mea masani ile vailaʻau namu. Ona o le lautele aoga o le lactoperoxidase faʻaopopo, ua manaʻomia tele lona siʻitia i le aluga o tausaga, ma o loʻo tupu pea.
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O le a le Lactoperoxidase?

Lactoperoxidase e na o se peroxidase enzyme e gaosia mai le mucosal, susu, ma faʻataʻamilomilo, lea e galue o se masani aneti-siama vailaʻau. I tagata soifua, o le lactoperoxidase enzyme e faʻatauina e le LPO gene. O lenei enzyme e masani ona maua i mamalia, e aofia ai tagata, isumu, povi, kamela, pafalo, povi, 'oti, Ilama, ma mamoe.

Galuega Lactoperoxidase:

LPO o se sui sili ona aoga antimicrobial sooupu. Lactoperoxidase faʻaaogaina, o le mea lea, faʻavae i luga o lenei faʻavae. Lactoperoxidase talosaga o lea e maua tele i taumafa faasao, ophthalmic fofo, ma teuteu faamoemoega. Lea foi, lactoperoxidase efuefu ua faʻaaogaina i manua ma nifo togafitiga. E le gata i lea, o le LPO o se mea aoga aneti-viral ma anti-tumor. Talanoaina i lalo e sili atu lactoperoxidase faʻaaogaina:

i. Tue kanesa o le susu

Kanesa Lactoperoxidase pulega puleaina e fesoʻotaʻi ma lona tomai faʻavasegaina estradiol. O lenei faʻamamafa e oʻo atu ai ile faʻamaʻi oona ile sela o le susu. Ole galuega ole Lactoperoxidase ole mafuaʻaga lea ole mafuaʻaga ole gaioiga e mafua ai le taumafaina o le okesene ma le faʻaputuputu o le intracellular hydrogen peroxide. O se faʻaiuga o nei faʻaiuga, e fasiotia lelei ai e le LPO sela o le tino in vitro. E faʻapipiʻi foi macrophages faʻaalia LPO e faʻaleaga ai kanesa sela, fasiotia ai.

ii. Aneti-siama aafiaga

O le enzim LPO o se vailaau faʻalenatura o le le-faʻamalosia ola puipuia puipuiga mamalia ma faʻaaogaina le faʻaogaina o le thiocyanate i totonu o le antibacterial hypothiocyanate. LPO mafai ona taofia le tuputupu aʻe o se lautele lautele o meaola ninii e ala i se enzymatic tali e aofia ai thiocyanate iona ma le hydrogen peroxide o cofactors. Ole gaioiga ole antimicrobial o le LPO e faʻavae ile faʻatulagaina o hypothiocyanite ionia e ala ile faʻagaioiga o enzymes. Iona Hypothiocyanite e mafai ona tali atu i le siama siama. Latou te faʻatupuina foʻi le faʻalavelave i le galuega o ni metabolic enzymes. Lactoperoxidase e fasiotia Gram-leaga siama ma puipuia le tuputupu ae ma le atinaʻega o Gram-lelei siama.

iii. Lactoperoxidase i mea teuteu

O le tuʻufaʻatasiga o le lactoperoxidase paula, kulukose, thiocyanate, iodide,

ma kulukose oxidase, ma ua iloa e aoga tele i teuteuga teuteu.Lactoperoxidas-02

iv. Lactoperoxidase i le susu Puipuiga

Le tomai o le lactoperoxidase i le tausiga o le mama mama o le susu susu mo se taimi atofaina ua faʻavaeina i le tele o fanua ma faʻataʻitaʻiga suʻesuʻega na faʻatinoina i isi nofoaga faʻaitulagi. Lactoperoxidase preservative e mafai ona faʻaaogaina e puipuia lelei ai susu susu e maua mai ituaiga eseese. Ole a le aoga ole auala ole faalagolago lea ile tele o itu. O mea nei e aofia ai le susu i le taimi o togafitiga, ituaiga o siama microbiologis, ma le aofaʻi o le susu.

E maua e le Lactoperoxidase se siama i le susu o le susu susu. Suesuega faʻamaumauga ma poto masani mai faʻataʻitaʻiga moni faʻaalia ai lactoperoxidase mafai ona faʻaogaina tala atu o le vevela tapulaʻa o (15-30 tikeri Celsius) o lo o taua i le codex taiala o le 1991. I le pito sili ona iʻu o le fua o le vevela, eseese suʻesuʻega faʻaalia ai o le faʻaaogaina o lactoperoxidase mafai ona tuai le psychrotrophic susu siama tuputupu ae ma faʻapena tuai le faʻaleagaina o le susu mo le tele o aso faʻatusatusa i le faʻamaloloina na o ia. E taua tele le maitauina o le mafuaʻaga o le faʻaaogaina o le lactoperoxidase e le o le faʻamamaluina o le susu mo le taumafaina ae ia puipuia lona tulaga lelei muamua.

Faʻataʻitaʻiga lelei le tumama i le gaosiga o le susu e taua tele i le aoga o le lactoperoxidase ma le lelei o le susu susu. O le saogalemu ma le fou o le susu e mafai ona faʻatinoina e ala i le tuʻufaʻatasia o vevela togafitiga o le susu ma lelei faʻamama faiga tuʻufaʻatasi e faʻaaogaina le lactoperoxidase.

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v. Isi galuega

Suesuega ua faʻaalia e faʻaopopo atu i le iai o aafiaga antivirus, Lactoperoxidase mafai foi ona puipuia ai sela a manu mai le tele o faʻaleaga ma peroxidation, ma o se mea taua foi o le puipuiga puipuiga mai faʻamaʻi patogenic meaola i totonu o le faʻaogaina o le pepe fou pepe.

Lactoperoxidase System

O le a le Lactoperoxidase System?

Lactoperoxidase system (LPS) o loʻo faia i ni vaega se tolu, e aofia ai lactoperoxidase, hydrogen peroxide, ma thiocyanate (SCN ¯). Ole Lactoperoxidase system e iai aneti-siama gaioiga pe a fai e galulue faʻatasi nei vaega e tolu. I le faʻaogaina moni-olaga, pe a fai o le tuʻufaʻatasia o se mea taua i le faiga e le lava, e tatau ona faʻaopopoina e mautinoa le aneti-siama aafiaga, lea ua lauiloa o le LPS faʻamalosi. I totonu o latou, o le lactoperoxidase faʻapitoa e le tatau ona i lalo ifo o le 0.02 U / mL.

Ole faʻatulagaina ole lactoperoxidase ile susu ole 1.4 U / mL, e ono faʻamalieina ai lenei manaʻoga. SCN ¯ o loʻo avanoa lautele i faʻalilolilo ma aano o manu. I le susu, o le maualuga o thiocyanate e laʻititi lava i le 3-5 μg / mL. Ole tapulaʻa lea o mea mo le Lactoperoxidase system gaioiga. Na fautuaina e faapea o le thiocyanate e manaʻomia e faʻagaioia ai le lactoperoxidase system e lata i le 15 μg / mL pe sili atu foi. O le mafuaʻaga lea e tatau ai ona tatou faʻaopopoina lenei exogenous thiocyanate e faʻagaoioia ai le lactoperoxidase system. Ole hydrogen peroxide o loʻo iai i totonu ole susu, na na o le extruded, e naʻo le 1-2 μg / Ml, ma o le gaioiga o LPS e manaʻomia 8-10 μg / mL o le hydrogen peroxide. Ole mafuaaga lea ole hydrogen peroxide e tatau ona maua mai fafo.

Ole lactoperoxidase system e taua tele lona sao i le puipuiga o le tino, e mafai ona fasia siama i le susu ma mukosa faʻafuaseʻi ma ono i ai ni togafitiga aoga.

I meaai ma le soifua maloloina oloa faʻaopopo, faʻaopopoga poʻo le faʻateleina o le lactoperoxidase faiga i nisi taimi faʻaaoga mo le puleaina o siama.

E faapefea ona galulue?

O le LPS e aofia ai le gaosiga o le aneti-siama vailaʻau mai le SCN ¯ faʻamaʻi e le LPO i le i ai o le hydrogen peroxide. Le lactoperoxidase na fai mai antimicrobial gaioiga e masani ona maua i le tele o vai vai a le tino pei o sua o le gastric, loimata, ma suavai. O mea taua e lua mo le antimicrobial system, o le hydrogen peroxide ma thiocyanate, o loʻo iai i totonu o le susu i fula eseese, faʻalagolago i ituaiga o meaola ma meaʻai tuuina atu.

I le susu fou, o le antimicrobial gaioiga e vaivai ma nofo mo le itiiti ifo 2 itula talu mai le susu na o aofia ai tulaga sili ona lelei o le hydrogen peroxide ma thiocyanate ion. Ua faʻaopopoina le Thiocyanate o loʻo faʻapipiʻiina i le 2 eletise eletise e maua ai le hypothiocyanite

Thiocyanate galue o se kofactor mo le Lactoperoxidase system. O se taunuuga, o le fuainumera o le oxidized sulfhydryls i le aotelega e tutoatasi mai thiocyanate ion i le umi

  1. O loʻo avanoa Thiol moiety
  2. E le ova le Thiocyanate
  • E lava le hydrogen peroxide o loʻo iai
  1. Thiocyanate e leʻo faʻaopoopoina i totonu o se manogi amino acid

O se faʻaiuga, o le thiocyanate e toe faʻatinoina le aneti-siama faʻamaʻi o le lactoperoxidase system i le susu fou. O lenei faʻalautele le umi ole ola o fou susu i lalo o tulaga vevela mo le fitu i le valu itula.

Lactoperoxidase Talosaga / Faʻaaogaina

i. Anti-microbial Action

O le aneti-microbial gaioiga o le lactoperoxidase faiga o loʻo vaʻaia i le bactericidal ma bacteriostatic gaioiga o nisi meaola ninii maua i le susu susu. O lona siama ole faʻamaonia o loʻo galue i totonu o le vaega thiol o loʻo maua i luga o le microbial cell 'plasma membrane e faʻaaogaina. O lenei iʻuga e mafua ai le faʻatamaʻia o le fausaga seleni plasma faʻatamaʻi atu ai i le tatalaga o polypeptides, potassium ions, ma le amino acids. O le taliaina o purines ma pyrimidines, kulukose, ma amino acid e sela ua taofia. Synthesis o DNA, RNA, ma porotini o loʻo taofia foi.

Eseese siama faʻaalia faʻailoga tikeri o le mafaufau lelei i le lactoperoxidase system. O siama leaga-siama, pei o Salmonella, Pseudomonas, ma Escherichia coli, ua taofia ma fasiotia. Ole siama ole Lactic acid ma le Streptococcus e naʻo le faʻatagaina. Faʻaleagaina o nei siama e le lactoperoxidase faiga mafua ai le ova o nisi o meaʻai, faʻalavelaveina siama mai le aveina i meaʻai, ma o lenei mea e mafua ai le faʻaititia po o le oti o siama.

ii. Togafitiga o paradentosis, gingivitis, ma fasiotia sela sela

LPS ua talitonuina e aoga i le togafitiga o le gingivitis ma le paradentosis. LPO ua faʻaoga i le gutu e vili ai ina ia tuʻu ifo i lalo o siama siama ma, o le mea na tupu, o le siama na gaosia e nei siama. O loʻo faʻaalia e aoga le faʻatonutonuina o le tino lactoperoxidase ma le kulukose oksidase i le faʻaleagaina ma mafua ai ona fasiotia o sela sela i vitro. E faʻapea foi i macrophages faʻaalia i le lactoperoxidase faiga, e faʻagaioia e faʻaleagaina ma fasiotia ai sela kanesa.

iii. Tautoga Tautino

Eseʻesea suʻesuʻega falemaʻi na faʻamatalaina le aoga o le LPS i mea fulunifo ua faʻamaumauina. Ina ua maeʻa faʻaalia faʻaalia, faʻaaoga le fuaina o faʻataʻavaleina tulaga faʻatapulaʻa, o lopa tisifo lactoperoxidase o loʻo i ai amyloglucosidase (γ-amylase) ei ai aafiaga aoga i le tausiga tuufofoga. Enzymes pei o kulukose oxidase, lysozyme, ma lactoperoxidase o loʻo faʻaliliu saʻo mai le mea fulunifo i le pellicle.

I le avea ai ma vaega o le pellicle, o nei enzymes e matua malosi gaioiga catalytically. E le gata i lea, o le LPS e i ai le aoga aoga o le puipuia mai le amata atu i fanau laiti e ala i le tuʻu i lalo o le numera o kolone lea e fausia e le cariogenic microflora a faʻateleina ai le mafaufauina o thiocyanate.

Faatasi ai ma xerostomia gasegase, lactoperoxidase mea fulunifo e sili maualuga pe a faʻatusatusa atu i fluoride mea fulunifo pe a sau i le faʻavae plaque. E le faʻatapulaʻaina le faʻaaogaina o le LPS i le periodontitis ma learies. Ole tuʻufaʻatasiga o lactoperoxidase ma lysozyme e mafai ona faʻaaogaina ile susunuina ole gutu togafitiga.

A tuʻufaʻatasia LPS ma lactoferrin, o lenei tuʻufaʻatasiga e faʻatatau i le halitosis. A tuʻu faʻatasi le LPS ma le lysozyme ma le lactoferrin, e fesoasoani le LPS i le faʻaleleia atili o le xerostomia ia faʻailoga. Faʻapena foi, gels ma lactoperoxidase faiga fesoasoani e faʻaleleia atili ai faʻamaʻi kanesa pe a faʻalavelaveina le gaosi lelea ona susulu.

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iv. Siʻitia o le tino puipuia

Lactoperoxidase antimicrobial gaioiga taua tele galuega i le puipuiga puipuia. O le Hypothiocyanite o se mea gaioi na gaosia e le lactoperoxidase gaioiga i luga o le thiocyanate. O le hydrogen peroxide e maua mai i le Duox2 porotini (faʻalua oxidase 2). Ua faʻaitiitia le faʻaitiitia o le Thiocyanate i tagata mamaʻi o le cystic fibrosis. O lenei iʻuga i le faʻaititia i le gaosiga o le antimicrobial hypothiocyanite. Sa fesoasoani i lenei i se tulaga maualuga atu o faʻamaʻi pipisi i luga ole ea.

Ua taofia e le LPS le helicobacter pylori. Ae faʻaalia i le tino o aʻa, LPS e faʻaalia ai se vaivai o le anti-bacterial effects. E le osofaʻia e le LPS DNA ma e le o le mutagenic. Peitaʻi, i lalo o tuʻuga faʻapitoa, o le LPS e ono faʻatupuina ai le faʻamamaʻi. E faʻamaonia o LPO i le thiocyanate o loʻo i ai e mafai ona faʻatupu ai le sitotoxic ma bactericidal aafiaga o le hydrogen peroxide i lalo o faʻapitoa tulaga, e aofia ai pe a H2O2 o iai i le fefiloi o le tali sili atu o thiocyanate.

I se faʻaopopoga, ona o lona malosi ma aoga antibacterial meatotino ma le maualuga o le malosi o le vevela, o loʻo faʻaogaina e avea o se antibacterial sooupu mo le faʻaitiitia o siama nuu i le susu poʻo oloa susu ma o se faʻailoga o le susu ultra-pasteurization. E ala ile faʻaaogaina o le lactoperoxidase system, o le umi o le ola o le faʻaʻaisa susu susu mafai foʻi ona faʻalautele.

Ma, o le hypothiocyanate e gaosia e le lactoperoxidase e mafai ona faʻaaogaina e taofia ai le herpes simplex virus ma le human immunodeficiency virus.

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E saogalemu mo le soifua maloloina o tagata ma manu?

E sefululima tausaga o suʻesuʻega o fanua i le atinaʻe ma atiaʻe atunuʻu na faia ma suʻesuʻe e le FAO / WHO JECFA (Joint Experts Committee on Food Additives). Ina ua maeʻa nei suʻesuʻega loloto ma tele faʻamaeʻaina, o le faʻaaogaina o le lactoperoxidase system i le susu faasaʻo na faʻamaonia e le FAO / WHO JECFA (Komiti Faʻapitoa mo Meaʻai Pea). Ua taʻua foi e le au atamamai, lenei auala e saogalemu mo le soifua maloloina o tagata ma manu.

O le LPS o se natura faʻavae o le faʻamalositino sua suavai ma saliva i tagata ma, o lea, saogalemu pe a faʻaaogaina e usitaʻia le Codex Alimentarius Komisi Taʻiala. O lenei auala e le aʻafia ai le faʻafetau manu. E mafua lenei mea ona o le togafitiga e faia naʻo le susu mai le teat.

iʻuga

E iloga mai la matou talanoaga o le lactoperoxidase ma le lactoperoxidase system e matua aoga ma e aoga tele i le lautele lautele o talosaga. Afai o lo o e saili atu e avea ma atoatoa lactoperoxidase faʻatau mo lau suʻesuʻega poʻo le atinaʻeina o vailaʻau, le toe vaai O loʻo ia i matou le tomai e faʻagaioia ai le tele o poloaiga lactoperoxidase i le taimi puʻupuʻu e mafai ai ma lafo atu i Amerika, Europa, Kanata, ma isi vaega o le lalolagi. Aua le saoloto e faʻafesoʻotaʻi mai matou mo nisi faʻamatalaga.

mau faasino

  1. Jantschko, PG Furtmüller, M. Allegra et al., "Redox ogatotonu o peroxidases o laau ma mamalia: o se faʻatusatusaina sementara-kinetik suesuega o lo latou tali atu agai i le indole derivatives," Archives of Biochemistry and Biophysics, vol. 398, nu. 1, itu. 12–22, 2002.
  2. Tenovuo JO (1985). "O le peroxidase faiga i faʻamalamalamaga tagata." I Tenovuo JO, Pruitt KM (eds.). Le Lactoperoxidase system: kemisi ma ola faʻatatau taua. Niu Ioka: Dekker. itu. 272.
  3. Thomas EL, Bozeman PM, Aʻoaʻo DB: Lactoperoxidase: fausaga ma mea totogia. Peroxidases i kemisi ma biology. Faatonutonu e: Everse J, Everse KE, Grisham MB. 1991, Boca Raton, FL. CRC Press, 123-142.
  4. Wijkstrom-Frei C, El-Chemaly S, Ali-Rachedi R, Gerson C, Cobas MA, Forteza R, Salathe M, Conner GE (Aukuso 2003). “Lactoperoxidase ma le puipui o tagata e puipuia le puipuia”. Am. J. Respir. Feaveai Mol. Biol. 29 (2): 206–12.
  5. Mikola H, Waris M, Tenovuo J: Inhibition of herpes simplex virus type 1, respiratory syncytial virus, ma echovirus ituaiga 11 e le peroxidase-faʻatupuina hypothiocyanite. Antiviral Res. 1995, 26 (2): 161-171.
  6. Haukioja A, Ihalin R, Loimaranta V, Lenander M, Tenovuo J (Setema 2004). "Mautinoa o Helicobacter pylori i se tulaga puipuia puipuiga, o le lactoperoxidase faiga, i le buffer ma i totonu o le tagata atoa ludah". Lautusi o Medical Microbiology. 53 (Pt 9): 855–60.

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